TPO Antibody (anti-Thyroid Peroxidase Antibody) BioAssay ELISA Kit (Rat)-TPO Antibody (anti-Thyroid Peroxidase Antibody) BioAssay ELISA Kit (Rat)代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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中文名称: TPO Antibody (anti-Thyroid Peroxidase Antibody) BioAssay ELISA Kit (Rat)

英文名字: TPO Antibody (anti-Thyroid Peroxidase Antibody) BioAssay ELISA Kit (Rat)

供应商: USBiological

产品货号: 384852

产品报价: ￥1/96Tests

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: The TPO Antibody (anti-Thyroid Peroxidase Antibody) BioAssay ELISA Kit (Human) is a quantitative sandwich assay for the detection of TPO Antibody in human serum, plasma and other biological fluids.Sensitivity:<0.469IU/mlRange:0.781-50IU/mlPrecision: Intra-Assay CV: <8%Inter-Assay CV: <10%Specificity:This assay has high sensitivity and excellent specificity for detection of rat TPO Antibody. No significant cross-reactivity or interference between TPO Antibody and analogues was observed.Assay Principle:The microtiter plate provided in this kit has been pre-coated with antigen (Thyroid Peroxidase). Standards and samples are added to the appropriate microtiter plate wells followed by biotinylated antigen (biotinylated Thyroid Peroxidase). Streptavidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. TMB substrate solution is added, after which the enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the resulting color measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TPO Antibody in the sample is then determined by comparing the O.D. of the sample to the standard curve.Kit Components:*384852A: Microtiter Strips, 8x12 wells.*384852B: Standard, 2 vials384852C: Sample/Standard Dilution Buffer, 1x20ml384852D: Thyroid Peroxidase-Biotin (Concentrated), 1x120ul384852E: Antigen Dilution Buffer, 1x10ml*384852F: Streptavidin-HRP Conjugate (SABC), 1x120ul384852G: SABC Dilution Buffer, 1x10ml*384852H: TMB Substrate, 1x10ml384852J: Stop Solution, 1x10ml384852K: Wash Buffer (25X), 1x30mlStorage and Stability:Store unopened *384852A at 4ºC; store at -20ºC once opened. Store unopened *384852B at 4°C; once reconstituted store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store *384852F and *384852H in the dark at 4°C. Store all other components at 4°C. Unused kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.Assay Summary:1. Wash plate 2 times before adding standards, samples and control (zero) to wells.2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37°C 3. Aspirate the liquid and wash plate 3 times.3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37°C4. Aspirate the liquid and wash plate 3 times.5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C6. Aspirate the liquid and wash plate 5 times.7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37°C8. Add 50ul Stop Solution. Read at 450nm immediately.9. Calculate results.

产品特点: 针对TPO Antibody (anti-Thyroid Peroxidase Antibody) BioAssay ELISA Kit (Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到TPO Antibody (anti-Thyroid Peroxidase Antibody) BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。