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中文名称: Angiopoietin-2 BioAssay ELISA Kit (Rat)

英文名字: Angiopoietin-2 BioAssay ELISA Kit (Rat)

供应商: USBiological

产品货号: 396562

产品报价: ￥1/96Tests

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.Sample Type:Cell lysates, cell culture supernates, serum, plasma (Heparin or EDTA).Intended Use:Sandwich High Sensitivity BioAssay ELISA kit for Quantitative Detection of Rat Angiopoietin-2.Sensitivity:<15pg/mlRange:156pg/ml-10,000pg/mlSpecificity:Recognizes natural and recombinant Rat Angiopoietin-2. There is no detectable cross-reactivity with other relevant proteins.Test Principle:This Rat Angiopoietin-2 BioAssay ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: Y19-F496) and test samples are added to the wells, a biotinylated detection specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Angiopoietin-2 amount of sample captured in plate.Kit Components:96-well plate precoated with anti-Rat Angpt2 antibody, 1x plateLyophilized recombinant Rat Angpt2 standard, 2x10ng/tubeBiotinylated anti-Rat Angpt2 antibody, 1x130ul (dilution 1:100)Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)Sample diluent buffer, 1x30mlAntibody diluent buffer, 1x12mlABC diluent buffer, 1x12mlTMB color developing agent, 1x10mlTMB stop solution, 1x10mlPBS washing buffer Powder for 1000ml, 1x bufferStorage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对Angiopoietin-2 BioAssay ELISA Kit (Rat)该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到Angiopoietin-2 BioAssay ELISA Kit (Rat)产品后将其置于4°C/-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。