ApoE belongs to a group of proteins that bind reversibly with lipoprotein and play an important role in lipid metabolism. In addition to facilitating solublization of lipids, these proteins help to maintain the structural integrity of lipoproteins, serve as ligands for lipoprotein receptors, and regulate the activity of enzymes involved in lipid metabolism. Significant quantities of ApoE are produced in liver and brain and to some extent in almost every organ. ApoE is an important constituent of all plasma lipoproteins. It’s interaction with specific ApoE receptor enables uptake of chylomicron remnants by liver cells, which is an essential step during normal lipid metabolism. ApoE exists in three major isoforms; E2, E3, and E4, which differ from one another by a single amino-acid substitution. E3 is the most common isoform and is present in 40-90% of the population. Recombinant human ApoE3 is a 34.0 kDa protein containing 299 amino acid residues. This protein has a N-terminal His-tag.
序列
重组人载脂蛋白E3蛋白产品,又称Apolipoprotein E3, APOE, apolipoprotein, apolipoproteins.其对应NCBI基因号为348,NCBI基因别称为APOE;该基因种属来源为人源,对应Accession号为NM_000041.该蛋白产品又称Human ApoE4, ApoE4, Apo-E4, Apo E4, h-ApoE4, rh-ApoE4, recombinant human ApoE4, recombinant human ApoE4, Apo等.
来源宿主
提供的【ApoE3】重组人载脂蛋白E3产品的来源为大肠杆菌中重组表达.
溶解建议
提供的重组人载脂蛋白E3 产品为冻干粉末建议将其重新溶解于 5 mM 磷酸钠溶液 pH 7.8和0.5 mM DTT至浓度为0.1-1.0 mg/ml,后继实验可根据实验要求使用合适的水溶液进一步稀释.