Akt2 or Protein Kinase B β (PKBβ) is a serine/threonine kinase that is a member of the Akt family. Akt2 like the other Akt members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3, 4, 5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2. Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (3).
序列
Akt2,有活性的蛋白产品,又称Akt, RAC-alpha serine/threonine-protein kinase.其对应NCBI基因号为207,NCBI基因别称为AKT1源,对应Accession号为P31749.该蛋白产品又称Active Akt2, Akt2, human Akt2, active kinase, active kinases, Akt, recombinant Akt2, recombinant Akt, kinase activity assay, protein kinase, akt 2, akt-2, 等.
来源宿主
提供的【Akt2】Akt2,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.
溶解建议
提供的Akt2,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.