The RSK (ribosomal S6 kinase) family comprises growth factor-regulated serine/threonine kinases, known also as p90(rsk). RSK1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. Moller described the cloning and characterization of 3 genes encoding RSKs, and HU1 (also named RPS6KA1, or RSK1) cDNA encodes a predicted 735-amino acid protein containing 2 distinct consensus ATP-binding site sequences. Northern blot and RNase protection analyses detected an approximately 3.5-kb HU1 transcript in lymphocytes, skeletal muscle, liver, and adipose tissue (1). Zeniou determined the expression of the RSK1, RSK2, and RSK3 genes in various human tissues, during mouse embryogenesis, and in mouse brain (2). RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation (3).
序列
RSK1蛋白,有活性的蛋白产品,又称RSK, ribosomal s6 kinase (rsk), pp90RSK4.其对应NCBI基因号为3055,NCBI基因别称为HCK源,对应Accession号为PO8631.该蛋白产品又称rsk-1, rsk 1, Active RSK1, RSK1, RSK, human RSK1, active kinase, active kinases, recombinant RSK1, recombinant RSK, kinase activity assay, protein kinase等.
来源宿主
提供的【RSK1】RSK1蛋白,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.
溶解建议
提供的RSK1蛋白,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.