RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).
序列
RAF1蛋白,有活性的蛋白产品,又称RAF, RAF proto-oncogene serine/threonine-protein kinase , Proto-oncogene c-RAF.其对应NCBI基因号为6198,NCBI基因别称为PS6K源,对应Accession号为P23443.该蛋白产品又称raf-1, raf 1, Active RAF1, RAF1, RAF, human RAF, active kinase, active kinases, recombinant RAF1, recombinant RAF, kinase activity assay, protein kinase等.
来源宿主
提供的【RAF1】RAF1蛋白,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.
溶解建议
提供的RAF1蛋白,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.