The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice, Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Accumulating data support that the expression of Pim1 is mediated through activation of the JAK/STAT pathway. Some of the substrates of Pim1 include p21 Cip1, nuclear mitotic appartus protein, PTP-U2S and Socs-1. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis. Pim1 interacts with c-Myb via the DNA binding domain and regulates its transcriptional activity.
序列
Pim 1蛋白,有活性的蛋白产品,又称Pim, Serine/threonine-protein kinase Pim-1.其对应NCBI基因号为5595,NCBI基因别称为ERK1源,对应Accession号为P27361.该蛋白产品又称pim1, pim-1, Active Pim 1, Pim 1, Pim, human Pim, active kinase, active kinases, recombinant Pim 1, recombinant Pim, kinase activity assay, protein kinase等.
来源宿主
提供的【Pim 1】Pim 1蛋白,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.
溶解建议
提供的Pim 1蛋白,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.