Most of the effects of cAMP are mediated through the phosphorylation of target proteins on serine or threonine residues by the cAMP-dependent protein kinase (PKA). The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. The mammalian catalytic subunit has been shown to consist of three PKA gene products: C-α, C-β, and C-γ. Two PKA isoforms exist, designated types I and II, which differ in their dimeric regulatory subunits, designated RI and RII, respectively. Furthermore, there are at least four different regulatory subunits: RI-α, RI-β, RII-α, and RII-β. The cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The catalytic subunit C-β of PKA (PKAcb) is a member of the Ser/Thr protein kinase family and is a catalytic subunit C-β of AMPK. Berube et al. assigned the PKAcb to human chromosome 1 by Southern blot analysis of somatic cell hybrids (1) and Simard et al located it to 1p36.1 by in situ hybridization (2).
序列
PKAcb蛋白,有活性的蛋白产品,又称PKA, cAMP-dependent protein kinase catalytic subunit alpha.其对应NCBI基因号为5566,NCBI基因别称为PRKACA源,对应Accession号为NM_002730.该蛋白产品又称Active PKAcb, PKAcb, PKA, human PKAcb, active kinase, active kinases, recombinant PKAcb, recombinant PKA, kinase activity assay, protein kinase等.
来源宿主
提供的【PKAcb】PKAcb蛋白,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.
溶解建议
提供的PKAcb蛋白,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.