Creasy identified a cDNA encoding STK3, which they called MST2. Sequence analysis indicated that the deduced 491-amino acid STK3 protein contains an N-terminal catalytic domain characteristic of STKs (1). Taylor also isolated cDNAs encoding STK3 (p63) and STK4 (p61) respectively. STK3 and STK4 share 94% amino acid identity in the catalytic domain and 78% identity overall (2). The yeast ’sterile 20’ (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. O’Neill used proteomic analysis of RAF1 signaling complexes to show that RAF1 counteracts apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2) (3). MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (2; 4). STK3 activation presumably allows cells to resist unfavorable environmental conditions.
序列
STK3蛋白,有活性的蛋白产品,又称STK, serine/threonine kinase 3, Mammalian STE20-like protein kinase 2, STE20-like kinase MST2, Serine/threonine-protein kinase Krs-1.其对应NCBI基因号为6714,NCBI基因别称为SRC源,对应Accession号为P12931.该蛋白产品又称STK-3, STK 3, Active STK3, STK3, STK, human STK3, active kinase, active kinases, recombinant STK3, recombinant STK, kinase activity assay, protein kinase等.
来源宿主
提供的【STK3】STK3蛋白,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.
溶解建议
提供的STK3蛋白,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.