FRK (fyn-related kinase) or Rak is a nuclear tyrosine kinase and member of the Src sub-family. Restricted expression of FRK is detected in a broad range of cell lines with highest levels in epithelial cells. Increased expression of FRK has been shown in breast and renal cell carcinoma cell lines. In addition the retinoblastoma tumor susceptibility gene product pRb associates with FRK in vitro and in vivo (1). Overexpression of FRK in β-cells from the pancreas increases the susceptibility of these cells to β-cell-toxic events (hallmark of Type I diabetes)(2).
序列
FRK蛋白,有活性的蛋白产品,又称FRK, fyn-related kinase, FYN-related kinase, Nuclear tyrosine protein kinase RAK, Protein-tyrosine kinase 5.其对应NCBI基因号为5582,NCBI基因别称为PRKCG源,对应Accession号为NM_002739.该蛋白产品又称Active FRK, FRK, human FRK, active kinase, active kinases, recombinant FRK, kinase activity assay, protein kinase等.
来源宿主
提供的【FRK】FRK蛋白,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.
溶解建议
提供的FRK蛋白,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.