MET is a proto-oncogene that encodes a transmembrane growth factor receptor which is a heterodimer of two disulphide linked chains of 50 kd (α) and 145 kd (β). MET is widely expressed in the kidney, brain, lung, skin, and embryonic tissue (1). Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian cancers and has been shown to be involved in tumor cell migration and invasion (2).
序列
MET蛋白,有活性的蛋白产品,又称MET, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Short name=SF receptor, Tyrosine-protein kinase Met.其对应NCBI基因号为5159,NCBI基因别称为PDGFRB源,对应Accession号为NM_002609.该蛋白产品又称Active MET, MET, human MET, active kinase, active kinases, recombinant MET, recombinant MET, kinase activity assay, protein kinase等.
来源宿主
提供的【MET】MET蛋白,有活性的产品的来源为Sf9昆虫细胞-杆状病毒中重组表达.
溶解建议
提供的MET蛋白,有活性的 产品为液态形式溶解于储存液(50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% 甘油)中.