Cytochrome b5a (CYB5A) is a membrane-bound hemoprotein which functions as an electron carrier for several membrane bound oxygenases like cytochrome P450 enzymes. Defects in the CYB5A gene (METHB-CYB5A) cause methemoglobinemia also known as CYB5A-related methemoglobinemia (a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of blood, cyanosis and hypoxia). BioVision’s human recombinant CYB5A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-108 a.a of Uniprot Accession# P00167) and having a molecular mass of 14.9kDa. CYB5A is fused to a 24 amino acid long His-tag at the N-terminus & purified by proprietary chromatographic techniques.
序列
重组人细胞色素B5A又称Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5.其对应NCBI基因号为1528,对应Accession号为P00167.
来源宿主
提供的【CYB5A】重组人细胞色素B5A蛋白产品来源为大肠杆菌中重组表达.
溶解建议
提供的重组人细胞色素B5A产品为溶液形式.溶解于20 mM Tris-HCl缓冲液中(pH 8.0, 20% glycerol, 0.1 M NaCl and 1 mM DTT )