Taq DNA Polymerase is a thermostable enzyme that replicates DNA and exhibits a half-life of 40 minutes at 95°C. Taq catalyzes the polymerization of nucleotides into duplex DNA in the 5’ –3’ direction in the presence of magnesium. The enzyme has an apparent molecular weight of 94,000 daltons by SDS-PAGE and exhibits 5’– 3’exonuclease activity. Taq is recommended for use in PCR and primer extension reactions at elevated temperature. The 10X Reaction Buffer provided has been optimized for amplification of both short and long PCR products.
序列
Taq DNA聚合酶蛋白产品,又称
DNA polymerase I, thermostable, DNA polymerase I, thermostable (EC 2.7.7.7) (Taq polymerase 1)
polA pol1.Taq DNA聚合酶产品又称Taq DNA Polymerase, Taq DNA, DNA Polymerase, Taq Polymerase, Taq等.
来源宿主
提供的【Taq DNA Polymerase】Taq DNA聚合酶产品的来源为大肠杆菌中重组表达.
溶解建议
提供的Taq DNA聚合酶 产品为液态形式溶解于20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50%甘油, 0.5% Tween 20和0.5% Nonidet P40溶液中.
保存建议
在您收到艾美捷发出的【Taq DNA Polymerase】Taq DNA聚合酶产品后,建议将其置于-80°C保存.