DKK-1 is a 25.8 kDa secreted protein functioning as antagonist of the canonical Wnt signaling pathway. It is a member of the dickkopf-related protein family characterized by two cysteine-rich domains separated by a linker region. Binding of DKK-1 to the LRP5/6 co-receptor inhibits the interaction with Wnt and by forming a ternary complex with transmembrane Kremen-1 or -2, promotes the internalization of LRP5/6
产品描述
Method
Sandwich ELISA, HRP/TMB, 12×8-well detachable strips
Sample type
Serum
Sample volume
20 µl / well
Assay time
2 h / 1 h / 30 min
Sensitivity
1.7 pmol/l (= 43.0 pg/ml)
Standard range
0 – 160 pmol/l (= 0 – 4102 pg/ml)
Conversion factor
1 pmol/l = 25.8 pg/ml (MW: 25.8 kDa)
Precision
In-between-run (n=9): ≤3 % CV
Within-run (n=5): ≤ 3 % CV
Cross-reactivity
Human DKK-1 only.
No cross-reactivity or interference with recombinant human DKK-2, DKK-4, Kremen-1, Kremen-2 or LRP-6 is observed.
Validation Data
See validation data tab for: precision, accuracy, dilution linearity, values for healthy donors, etc
Detection range
1.7 – 160 pmol/l (=43 – 4102 pg/ml)