【MIP 3】重组人巨噬细胞增殖蛋白-3-Recombinant Human Macrophage Inflammatory protein-3 (CCL23)代理/参数/厂家-蛋白标准品-开云·官方站线上登录平台入口

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背景资料: CCL23 （MIP-3） is a ligand for the CCR1chemokine receptor. CCL23 is one of several cytokine genes clustered on the q-arm of chromosome 17， in a locus containing several other CC chemokines. MIP-3 chemoattracts monocytes， resting T-lymphocytes and neutrophils， but not activated lymphocytes. Furthermore， it was shown that MIP-3 inhibits colony formation of bone marrow myeloid immature progenitors. MIP-3 is mainly expressed in lung and liver tissue， but can be also found in bone marrow and placenta， as well as in some cell lines of myeloid origin.
Alternative splicing of the CCL23 gene produces 2 mRNAs which encode a short （CK?8） and a long （CK?81） isoform of the MIP-3. CK?8 cDNA encodes a 120 amino acid residue precursor protein with a putative 21 a.a. residue signal peptide which is cleaved to generate a 99 a.a. residue mature CK?8 （a.a. 22-120）. Further N-terminal processing of the 99 a.a. residue variant can produce a 75 a.a. residue CK?8 （a.a. 46-120） which is considerably more active than the 99 a.a. residue variant.
MIP-3 may be involved in the malignant progression of certain human cancer cells which overexpress ErbB2 through the transactivation of ErbB2 tyrosine kinase. MIP-3 may also be involved in angiogenesis via upregulation of matrix metalloproteinase MMP-2 expression.

溶解建议: 提供的人巨噬细胞炎症蛋白-3（MIP 3）产品为经0.2um孔径过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人巨噬细胞炎症蛋白-3（MIP 3）的20mM PB， pH 7.4， 150mM NaCl溶液中过滤灭菌并冻干产生的.我们建议加无菌的18MΩ-cm水以配制工作储存液到不低于100ug/ml，后继实验过程中可根据实验要求进一步稀释.

保存建议: 人巨噬细胞炎症蛋白-3（MIP 3）冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存.重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存.请避免反复冻融.

其他: ProSpec 是一家全球著名细胞因子蛋白生产和研发的公司，具有20多年的生产经验，专注于蛋白（重组及合成）生产研发。我们的核心产品包括：干扰素、白介素、骨蛋白、肿瘤坏死因子、成纤维生长因子、瘦素、干细胞因子、催乳素、表皮生长因子、胰岛素样生长因子、神经生长因子、集落刺激因子、激素、酶、病毒抗原以及各种重组蛋白等。领先的生产工艺和规模使其可以提供毫克到克级蛋白，价格优于同类公司。绝大部分产品是天然成熟型蛋白，而不含有标签蛋白。考虑到大部分研究者希望能更灵活地配制蛋白溶液，ProSpec-Tany绝大部分产品没有添加保护剂或盐，这就对工艺提出了更高的要求。同时，ProSpec-Tany绝大部分产品为冻干粉，因此易于运输和保存。