中文名称

Anti-Host Cell Protein (HCP) Antibody Production, Option B - CUST93C

英文名字

Anti-Host Cell Protein (HCP) Antibody Production, Option B - CUST93C

供应商

Rockland

产品货号

CUST93C

产品报价

￥询价/1Each

产品说明书

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购买方式

90%产品中国有现货库存。银行转账、电汇、支票、现金，在线支付宝及网银支付，或直接与我们电话联系400-6800-868

产品新闻

背景资料

Complete service includes immunizing two different host species for anti-HCP antibody production to control for host species variability. Client selects two host animals: rabbit, chicken or sheep. Rabbits are valued as host animals due to the volume of historical data. Chicken/avian host show a varied pattern of immune response to mammalian HCP preparations especially CHO cell derived. Sheep may show greater immune sensitivity and yield larger volumes of antisera. HCP preparation is immunized in the two selected hosts. Project includes: 10 RABBIT or CHICKEN hosts or 3 SHEEP hosts, 90 days daily maintenance EACH, 10 Pre-bleeds EACH (up to 5 mL each), 20 Test bleeds EACH (up to 5 mL each), 8 to 20 production bleeds EACH (4 production bleeds from the 5 best responding RABBITS or CHICKENS or 4 production bleeds from the 2 best responding SHEEP) of up to 25 mL each (65 mL for SHEEP) or up to 500 mL). Only host animals showing reactivity to a broad range of proteins by size are selected for production. Terminal bleeds (1 each from the best responding 5 RABBITS or CHICKENS or 2 SHEEP of 100 mL each or up to 500 mL), western blotting of all test bleeds, production bleeds and terminal bleeds and against one (1) HCP lysate provided by client. After selection, host animal production bleeds are pooled and then purified by a method equivalent to Protein A chromatography (7S fractionation or IgY purification from eggs) up to 1.0 L of qualified antiserum. Vialing and lyophilization of up to 100 glass vials. Final qualification of final product by western blotting against provided HCP lysates. Summary report (1 page) with digital copy of finished product western blot and SDS-PAGE. Package charge for shipping of finished product is included.

产品描述

Complete service includes separation of the host cell protein into high molecular weight (HMW) and low molecular weight (LMW) fractions by ultra filtration, 10 RABBIT host animals, 90 days RABBIT daily maintenance, 10 RABBIT Pre-bleeds (5 mL each), 10 RABBIT test bleeds (5 mL each), 20 RABBIT production bleeds (4 production bleeds from the 5 best responding animals of 25 mL each or up to 500 mL), 5 Rabbit terminal bleeds (1 each from the 5 best responding animals of 100 mL each or up to 500 mL), western blotting of all test bleeds, production bleeds and terminal bleeds and against one (1) HCP lysate provided by client, Protein A purification of up to 1.0 L of qualified antiserum, vialing and lyophilization of up to 100 glass vials. Final qualification of final product by western blotting against provided HCP lysate. Summary report (1 page) with digital copy of finished product western blot and SDS-PAGE. Package charge for shipping of finished product is included.

产品形式

该Anti-Host Cell Protein (HCP) Antibody Production, Option B - CUST93C的详细信息查看Rockland提供的产品说明书

保存建议

该Anti-Host Cell Protein (HCP) Antibody Production, Option B - CUST93C的详细信息查看Rockland提供的产品说明书

其他

Rockland成立于1962年，是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物。Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。

注意

该页面的中文产品信息的翻译，仅供参考。准确的产品信息请以厂家的英文说明书为准。下单前，请浏览说明书确认。

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