Human Cartilage Intermediate Layer Protein 2 (CILP2) ELISA Kit
供应商
Biomatik
产品货号
EKU02972
产品报价
¥询价/96T
产品说明书
购买方式
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产品新闻
背景资料
This assay has high sensitivity and excellent specificity for detection of Cartilage Intermediate Layer Protein 2 (CILP2). No significant cross-reactivity or interference between Cartilage Intermediate Layer Protein 2 (CILP2) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人软骨中间层蛋白2(CILP2)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.