Human Macrophage Derived Chemokine (MDC) ELISA Kit
供应商
Biomatik
产品货号
EKU05740
产品报价
¥询价/96T
产品说明书
购买方式
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产品新闻
背景资料
This assay has high sensitivity and excellent specificity for detection of Macrophage Derived Chemokine (MDC). No significant cross-reactivity or interference between Macrophage Derived Chemokine (MDC) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 (PD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 (PD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 1 (PD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Programmed Cell Death Protein 1 (PD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该人巨噬细胞衍生趋化因子(MDC)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.