Rat Secreted Frizzled Related Protein 2 (SFRP2) ELISA Kit
供应商
Biomatik
产品货号
EKU07218
产品报价
¥询价/96T
产品说明书
购买方式
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产品新闻
背景资料
This assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 2 (SFRP2). No significant cross-reactivity or interference between Secreted Frizzled Related Protein 2 (SFRP2) and analogues was observed.
产品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Parvalbumin (PVALB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Parvalbumin (PVALB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Parvalbumin (PVALB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Parvalbumin (PVALB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
产品特点
Double-antibody Sandwich
保存建议
Biomatik品牌厂家建议该大鼠分泌的卷曲相关蛋白2(SFRP2)ELISA试剂盒产品,Short term: 4°C; Long term: see manual.