DAB, a widely used chromogen for immunoperoxidase staining, is well accepted among pathologists because of its increased sensitivity and ability to give cleaner background as compared to amino ethylcarbazole (AEC). Specimens stained in DAB can be dehydrated, cleared, and mounted for permanent record keeping. Stable DAB/Plus is more sensitive and stable than traditional working DAB solutions. Peroxidase from the antibody detection system reacts with H2O2 substrate to degrade it, which then reacts with DAB, precipitating it at positive sites yielding a dark brown color.
产品描述
Stable DAB/Plus offers several noteworthy improvements and benefits as compared with traditional working DAB solutions. Stable DAB/Plus is much more sensitive, providing the cost-effective option of diluting the primary antibody. Being stable for 5 days (as opposed to 6 hours for traditional DAB working solutions), Stable DAB/Plus allows the user the convenience of making one working solution for the entire work week. Hazardous waste generation from spent DAB solution is also significantly reduced. Stable DAB/Plus is ideal for high volume labs and automated stainers.nReagents provided: Concentrated Amber-Colored DAB Chromogen Solution 5mL, Clear Stable DAB/Plus Substrate Buffer 200mL, Empty Mixing Dropper Bottle. Aliquot 20µL (1 drop) of DAB Chromogen Solution into 1mL of DAB/Plus Substrate Buffer in the Dropper bottle and mix thoroughly.
产品形式
Liquid
保存建议
Store at 2°-8°C away from light. Do not use product after the expiration date printed on vial. If reagents are stored under conditions other than those specified here, they must be verified by the user. Diluted reagents should be used promptly.