EZClick™全球蛋白质合成测定试剂盒（FACS /显微镜），绿色荧光-EZClick™ Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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细胞分析试剂盒

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中文名称: EZClick™全球蛋白质合成测定试剂盒（FACS /显微镜），绿色荧光

英文名字: EZClick™ Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence

供应商: Biovision

产品货号: K459-100

产品报价: ￥询价/100assays

产品说明书

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产品新闻

背景资料: 待更新

产品描述: e level of translation to ensure that production of specific cellular proteins is quickly turned on/off under specific conditions (heat shock, starvation, etc.). Protein synthesis is essential in cell growth, proliferation, signaling, differentiation or death; therefore, the identity and amount of the synthesized proteins are critical parameters in determining the physiological state of the cell. Methods enabling detection and characterization of nascent proteins, or changes in spatial and temporal protein expression/degradation patterns during disease, drug treatments or environmental changes are important tools in assessment of cytotoxicity. Biovision’s EZClick™ Protein Synthesis Monitoring Assay Kit utilizes a novel and robust chemical method based on an alkyne containing and cell-permeable analog of puromycin, O-Propargyl-puromycin (OP-puro). Once inside the cell, OP-puro stops translation by forming covalent conjugates with nascent polypeptide chains. Truncated polypeptides are rapidly turned over by the proteasome and can be detected based on a click reaction with the fluorescent azide. Unlike methionine analogs, OP-puro does not require methionine-free conditions and can be used to label nascent proteins directly in the cell culture. Our kit provides sufficient materials for 100 assays to detect nascent proteins synthesized under various physiological conditions, and Cycloheximide, an inhibitor of protein synthesis that serves as an experimental control

产品特点: Simple, fast, does not require lengthy incubation times

保存建议: -20

其他: BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.