Human Enolase α Inhibitor Screening Kit (Colorimetric)
供应商
Biovision
产品货号
K526-100
产品报价
¥询价/100assays
产品说明书
购买方式
银行转账、电汇、支票、现金,在线支付宝及网银支付,或直接与我们电话联系400-6800-868.
产品新闻
背景资料
待更新
产品描述
Enolase (EC 4.2.1.11), also called 2-Phospho-D-glycerate hydrolase, is a key enzyme in glycolysis. It converts 2-phosphoglycerate into phosphoenolpyruvate (PEP). It also catalyzes the reverse reaction, PEP to 2-phosphoglycerate under anabolic conditions during gluconeogenesis. In mammals, three genes encode for the Enolase α, β and γ isoforms. These isoforms combine together to form both homo and heterodimeric complexes resulting in five isozymes, three of which are commonly found in human tissues. Enolase α, also called enolase 1, exists in variety of tissues which can undergo glycolysis. Besides its role in glycolysis, increased Enolase α activity is associated with tumor invasion and metastasis; therefore, screening for novel and specific inhibitor of enolase α inhibitor may be of great interest for tumor treatment. In BioVision’s Human Enolase α Inhibitor Screening Kit, Enolase α catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is subsequently used to generate an intermediate product. The intermediate product stoichiometrically reacts with OxiRed™ probe generating a colorimetric signal (OD 570 nm). In the presence of Enolase α inhibitor, the reaction is impeded. An enolase α Inhibitor Control is included to compare the efficacy of the sample inhibitors. The assay is simple, fast, high-throughput adaptable and can be completed in less than 1 hr
产品特点
• Rapid and easy-to-use.
• High-throughput adaptable
• This assay kit can be used for initial screening of compounds as anti-Enolase