线粒体复合体I活性比色分析试剂盒-Mitochondrial Complex I Activity Colorimetric Assay Kit代理/参数/厂家-细胞分析试剂盒-开云·官方站线上登录平台入口

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细胞分析试剂盒

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中文名称: 线粒体复合体I活性比色分析试剂盒

英文名字: Mitochondrial Complex I Activity Colorimetric Assay Kit

供应商: Biovision

产品货号: K968-100

产品报价: ￥询价/100assays

产品说明书

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产品新闻

背景资料: 待更新

产品描述: Mitochondrial Complex I or NADH:ubiquinone oxidoreductase (EC 1.6.5.3) is the first and the largest complex of the Electron Transport Chain located in the mitochondrial membrane. It oxidizes NADH to NAD+ and transfers an electron to ubiquinone (also present in the inner mitochondrial membrane) converting it to ubiquinol. During this process, it transports protons across the inner mitochondrial membrane, helping to develop an electrochemical gradient. This process is very important for cellular respiration and adverse effects on Complex I activity can compromise mitochondrial respiration, which further leads to cellular stress. BioVision’s Mitochondrial Complex I assay kit is a fast and reliable method to determine the activity of complex I in isolated mitochondria. It is useful for respiration studies in isolated mitochondria and may be used to study effects of toxicants, drugs and other environmental conditions on mitochondrial complex I activity. This kit uses decylubiquinone, an analog of ubiquinone, as an electron acceptor that gets converted to decylubiquinol through the catalytic activity of Complex I. The Complex I dye that absorbs light at 600 nm in its oxidized form is used as a terminal electron acceptor that accepts electrons from decylubiquinol. Complex I activity is determined colorimetrically by recording the change in absorbance of reduced Complex I dye at 600 nm. Specific Complex I activity is obtained by subtracting the activity in presence of Complex I inhibitor rotenone from total activity. This kit can detect as low as 0.1 mU / well and is linear up to 7 mU / well.

产品特点: • Simple, rapid & convenient assay to measure Mitochondrial Complex I • Includes Inhibitor (Rotenone)

保存建议: -20

其他: BioVision的公司总部在美丽的旧金山湾地区，它是全球生命科学公司的领导者.BioVision在开发和研究细胞凋亡检测分析、代谢检测方面具有卓越的影响力.包括检测各种检测分析试剂盒、相关特色抗体、重组蛋白及细胞因子和酶等多种产品，以及其他创新的研究工具.其产品覆盖细胞凋亡研究、能量代谢、细胞/组织蛋白提取、氧化应激与损伤、代谢与肥胖症、细胞增殖与毒性、信号转导、干细胞研究等领域.作为多家知名品牌的中国总代理以及中国区域代理，艾美捷与BioVision一起为研究者们提供最好、最新的细胞生物学研究工具而不断扩大产品及服务质量.