肾组织裂解物通过在改良的RIPA缓冲液(150 mM氯化钠,50 mM Tris-HCl,pH 7.4,ImM乙二胺四乙酸,ImM苯甲基磺酰氟,1%Triton X-100,1%脱氧胆酸钠,0.1%十二烷基硫酸钠,μ5g/ml抑肽酶,5gμ/ml亮抑酶肽)中均质化制备。通过离心除去组织和细胞碎片。用Bio-Rad蛋白质测定法测定蛋白质浓度。将产物在含有50 mM DTT的lx SDS样品缓冲液(50 mM Tris-HCl pH 6.8,12.5%甘油,1%十二烷基硫酸钠,0.01%溴酚蓝)中煮沸5分钟。
产品描述
肾脏裂解物以含有50mM DTT的SDS样品缓冲液提供。
产品形式
该Kidney Lysate产品,产品形式为液体
保存建议
ProSci品牌厂家建议该Kidney Lysate产品,Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.