The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
序列
Met 1 - Gly 180
来源宿主
大肠杆菌
溶解建议
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保存建议
Lyophilized protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -70˚C or lower. Avoid repeated freeze-thaw cycles.