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中文名称: Resistin, Rat, BioAssay ELISA Kit

英文名字: Resistin, Rat, BioAssay ELISA Kit

供应商: USBiological

产品货号: R1585-04

产品报价: ￥1/1Kit

产品说明书

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背景资料: USBiological品牌是全球有名的抗原抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。武汉艾美捷科技作为USBiological品牌中国区域总代理，是行业中少有的致力于服务客户，帮助客户，且拥有独立的专业销售团队、技术支持团队、市场营销团队、进出口报关团队的高科技生物企业。可以为您提供及时的咨询响应，专业的产品和解决方案支持，稳健快捷的交货周期，优质放心的售后服务。我们致力于为您提供有价值的产品和服务，在意您的成功！

产品描述: Rat Resistin ELISA is a sandwich enzyme immunoassay for the quantitative measurement of rat resistin in serum.Features:• The total assay time is less than four hours. • The kit measures total serum (homodimeric) resistin.• Assay format is 96 wells.• Quality controls are recombinant rat resistin-based. No animal sera are used. • Standard is recombinant protein based.• Components of the kit are provided ready-to-use, concentrated or lyophilized.Test Principle: Standards, Quality Controls and samples are incubated in microplate wells pre-coated with polyclonal anti-rat resistin antibody. After 60 minutes incubation and washing, biotin-labelled polyclonal anti-rat resistin antibody is added and incubated for 60 minutes with captured resistin. After another washing, streptavidin-HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of resistin. A standard curve is constructed by plotting absorbance values against concentrations of Standards, and concentrations of unknown samples are determined using this standard curve.Kit Components:R1585-04A: Microtiter Plate, 1x96wellsR1585-04B: Master Standard, 1x1vialR1585-04C1: High Control, 1x1vial R1585-04C2: Low Control, 1x1vial R1585-04D: Pab (Biotin), 1x13mlR1585-04E: Streptavidin (HRP), 1x13mlR1585-04F: Dilution Buffer, 1x20mlR1585-04G: Wash Solution (10X), 1x100mlR1585-04H: Substrate Solution (TMB), 1x13mlR1585-04J: Stop Solution, H2SO4, 1x13mlStorage and Stability:Store all components at 4ºC. Unopened kit components are stable for 6 months. Upon reconstitution of R1585-04D, R1585-04E and R1585-04F, aliquot and store unused portions at -20°C. The aliquots are stable for 3 months at -20°C. Avoid repeated freeze thaw cycles. Other opened kit components are stable for 3 months at 4ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

产品特点: 针对Resistin, Rat, BioAssay ELISA Kit该产品的特点优势，欢迎查阅官网提供的产品说明书。

保存建议: 建议收到Resistin, Rat, BioAssay ELISA Kit产品后将其置于4°C/-20°C保存。

其他: Usbiological公司是美国著名的抗体和生化试剂供应商，生产世界上种类最多的抗体，用于Western Blot、免疫沉淀、免疫荧光、免疫组化和流式细胞术等多种检测方法。Usbiological公司现已拥有超过50,19234种抗体、抗原和生化产品，为科研用户提供了诸多超值选择。