7kb).Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly(A)-oligo(dT)12-18 as the template-primer.Standard cDNA Synthesis Conditions: 50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2ug radom hexamer,1-5ug RNA, 200units M-MLV RT. The reaction volume was 20µl and the incubation was 45 min at 42oC.5X Reaaction Buffer: 250mM Tris-HCl (pH8.3), 375mM KCl, 15mM MgCl2, and 50mM DTT.Applications:1. 1st strand cDNA synthesis.2. Primer extension.3. RT-PCR.Stability: Stable for 5 days at 10oC, for longer period of time store at -20oC. Please avoid freeze-thaw cycles.">
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