EvaGreen® Dye, 2000X in DMSO is offered for customers with specialized applications requiring a concentrated dye solution. Note: for most qPCR applications, we recommend our ready-to-use Forget-Me-Not™ EvaGreen® qPCR Master Mixes or EvaGreen® Dye, 20X in water. EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen® dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer. The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis. EvaGreen® dye and applications are covered under granted and pending US and international patents.
产品描述
EvaGreen®Dye,2000 X in DMSO适用于需要浓缩染料溶液的特殊应用。& nbsp; 注意:对于大多数qPCR应用,我们建议使用即用型勿忘我™EvaGreen®qPCR Master Mixes或EvaGreen®Dye,20 X水溶液。& nbsp; EvaGreen®染料是一种绿色荧光核酸染料,其特性使该染料可用于多种应用,包括qPCR和DNA熔解曲线分析、嗜热解旋酶依赖性扩增(tHDA)的实时监测和毛细管凝胶电泳。染料本身基本上是非荧光的,但在与dsDNA结合时变得高度荧光。DNA结合的染料具有非常接近荧光素(FAM)或SYBR绿色I的激发和发射光谱®,使其容易与配备有488 nm氩激光器或具有该区域波长的任何可见光激发的仪器兼容。EvaGreen®染料在热和水解方面都非常稳定,在常规处理过程中提供方便。EvaGreen®染料是非致突变性和非细胞毒性的,因为它是不可渗透的细胞膜,不像SYBR®Green I,它迅速进入细胞,是一种强大的突变增强剂。nbsp; EvaGreen染料的独特性质®使其在定量实时PCR(qPCR)应用中特别有用。与广泛使用的SYBR®绿色I相比,EvaGreen®染料通常对PCR的抑制性较小,并且不太可能引起非特异性扩增。因此,EvaGreen®染料可以以比SYBR Green I高得多的染料浓度使用®,从而产生用于液滴数字PCR、实时PCR和熔解曲线分析的高分辨率信号。nbsp; EvaGreen®染料和应用包括在已批准和正在申请的美国和国际专利中。