WB; IHC, Frozen; ELISA Western Blotting: This antibody detects multiple BDNF isoforms (14 kDa mature BDNF, 18 kDa isoform, 28 kDa BDNF dimer/truncated BDNF, 32 kDa proBDNF monomer) depending on sample application (human serum, cell lysate, tissue homogenate). Antibody also detects BDNF under non-reducing conditions (McLean NA, 2014).
ELISA: Detection only, 1:1000-1:5000 recommended.
Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide (HSDPARRGEL) as a part of human BDNF protein (aa: 129-138) conjugated to KLH has been used as the immunogen. The BDNF protein sequence is highly conserved amongst primates.
来源宿主
兔
反应性
This antiserum is known to recognise 大鼠, 小鼠, 人 BDNF,, is expected to react with BDNF from other species due to amino acid sequence homology.