WB; IHC, Frozen; ELISA; Neut, Neutralizing IHC, 1-site ELISA, WB, immunoblot, inhibition of biological activity. A concentration of 1-3 µg/mL is recommended for IHC, western blot and immunoblot, ELISA, inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
Native mouse beta NGF purified from submaxillary salivary gland (95% purity by PAGE)
来源宿主
绵羊
反应性
This antiserum is known to cross react with 小鼠, 大鼠, 人, avian NGF but not 牛 NGF.